International Journal of Diseases and Disorders

Full Length Research Paper

Metabolic control of lignocellulose degradation by lignocellulose-degradation microbial community MC1

Xiao-juan Wang, Peipei Li, Dongdong Zhang, Yanzhuan, Cao, Xiaofen Wang and Zong-jun Cui*

College of Agronomy and Biotechnology, Center of Biomass Engineering, China Agricultural University, Yuanmingyuan West Road, Haidian District, Beijing 100193, China.

Accepted 28 March, 2023

Abstract

This study was to reveal the limiting factors of lignocellulose degradation and further improve the potential degradation of microbial community MC1, by pH manual adjustment (pHMA) and culture medium partial replacement (CMPR) during the process of rice straw degradation. The results indicated degradation rate in control (5% rice straw) increased, ranging from 33.3 to 67.5% via pHMA method, and for treatment with CMPR which was elevated to 83.3%. Meanwhile, pH in control dropped to 5.4 and remained after 20 days of incubation, while pH increased to 6.9 in sample with pHMA and 6.5 in sample with CMPR. With molecular footprint technology, 9 bands undetected in previous work were discovered in MC1 from the DGGE profiles. The results of soluble products with two treatments demonstrated that CMPR technology could not only facilitate the degradation but also accumulated quantities of soluble products compared to pHMA method which decreased quantities of soluble products.

Key words: Lignocelluloses degradation, microbial community, pH manual adjustment, culture medium partial replacement.