ISSN 2756-3375
African Journal of Immunology Research ISSN 9431-5833 Vol. 7 (1), pp. 001-007, January, 2020. © International Scholars Journals
Full Length Research Paper
Evaluation of 11-hydroxy-∆8-THC-dimethylheptyl effects on cytokines profile and locomotor tests in experimental autoimmune encephalomyelitis
Mohammad Hossein Aarabi1, Mohammad Esmaeil Shahaboddin1*, Karim Parastouei1, Mitra Motallebi2, Akbar Jafarnejad1, Mehdi Mirhashemi1 and Gholam A. Hamidi3
1Department of Biochemistry and Nutrition, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R., Iran.
2Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R., Iran.
3Physiology Research Center, Department of Physiology and Pharmacology, Kashan University of Medical Sciences, Kashan, I. R., Iran.
Accepted 19 June, 2018
Abstract
One of the components that activate cannabinoids (CB) receptors is 11-hydroxy-∆8-THC-dimethylheptyl (HU-210). The present study was conducted to evaluate the effectiveness of HU-210 treatment to cytokines profile and locomotor tests in the experimental autoimmune encephalomyelitis (EAE) model in the mice. A total of 48 male C57BL/6 mice were placed in 8 therapeutic groups. Mice were immunized with myelin oligodendrocyte glycoprotein (MOG) 35–55. When EAE was observed¸ HU-210 was subcutaneously administered in 3 groups with different doses: 3,10,30 mg/kg. Control groups were treated to vehicle or HU-210. Drug or vehicle was given once on the following days: 1, 3, 5, 7, 9, 11, 13, 15, 17. During the study clinical scores of disease and balance beam test were analyzed. After 17 days of treatment, the animals sacrificed and, TNFα, IL-12, IL-4 level in serum was measured using enzyme-linked immunosorbent assay (ELISA). Our results showed that HU-210-treated mice had significantly less clinical score of EAE than non-treated EAE induced mice (p<0.05). Data revealed that dose 30 mg/kg HU-210 in EAE induced mice significantly decreased serum TNFα, IL-12 (p<0.05). Also, Serum IL-4 levels increased significantly in mice receiving 3, 10, 30 mg/kg dose of HU-210 (p<0.05). Present study revealed the beneficial effects of HU-210 in the model of EAE, and suggests its potential use as a drug for the treatment of multiple sclerosis (MS).
Key words: Experimental autoimmune encephalomyelitis, 11-hydroxy-∆8-THC-dimethylheptyl, cytokine, cannabinoids.