ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 11 (3), pp. 001-005, March, 2022. © International Scholars Journals
Full Length Research Paper
Effect of rosmarinic acid on estrogen, FSH and LH in female diabetic rats
Aleih Ghasemzadeh1, Arash Khaki1*, Laya Farzadi1, AmirAfshin Khaki2, Mehdi Marjani3, Hamidreza Ahmadi Ashteani4, Bushra A. Hamdi5, Elham Ghadamkheir1, Meisam Naeimikararoudi1 and Elaheh Ouladsahebmadarek1
1Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Anatomical Sciences, Tabriz University of Medical Sciences, (NPMC), Tabriz, Iran.
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
4Department of Biochemistry and Nutrition, Zanjan University of Medical Sciences, Iran.
5Department of Pharmacognosy, Pharmacy College, Hawler, Medical University, Kurdistan - Region, Iraq.
Accepted 02 August, 2021
Abstract
Rosmarinic acid from the plants quenched superoxide radicals from xanthine oxidase and inhibited cyclooxygenase I and II enzymes. Antioxidants have essential effect on diabetes. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat (n=40) were allocated into three groups, control group (n=10), rosmarinic acid (Ro) group that received 5 mg/rat (gavage) (n=10) and diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided into two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus 5 mg/rat Ro daily for 4 weeks, respectively. However, the control group just received an equal volume of distilled water daily (gavage). Diabetes was induced by a single (IP) injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28 days after inducing, diabetic 5 cc of blood were collected for sex hormones, TAC and MDA levels from the whole groups analysis. Level of MDA significantly decreased in group that has received 5 mg/rat of Ro (P<0.05) in comparison to experimental groups and sex hormones, and TAC significantly increased in groups that received Ro (P<0.05). Since in our study, 5 mg/rat of Ro, have significantly preventive effect on diabetic disorder in female sex hormone, so it seems that using it can be effective for treatment in diabetic Rat
Key words: Rosmarinic acid, diabetic, female sex hormone, streptozotocin.