ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 14 (4), pp. 001-007, April, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Impact of Oxytetracycline on Bacterial Load in Cultured Labeo rohita
Md. Abul Kashem1, Md. Naim Uddin2, Mohammad Mosarof Hossain1, Md. Tawheed Hasan1, Syed Ariful Haque3, Mohammed Nurul Absar Khan4, Mohammed Masud Hassan5 and Ferdaus Mohd. Altaf Hossain6
1Faculty of Fisheries, Sylhet Agricultural University,
2 Dept. of Fisheries Technology, Bangladesh Agricultural University
3Dept. of Fisheries Technology, Sheikh Fajilatunnesa Mujib Fisheries College
4Faculty of Fisheries, Chittagong Veterinary and Animal Sciences University,
5Noakhali Integrated Agro Industries Ltd. Noakhali Sadar, Noakhali, Banglladesh.
6Faculty of Veterinary & Animal Science, Sylhet Agricultural University, Bangladesh.
Accepted 03 March, 2025
The study was aimed to outline the anti biogram profiling of oxytetracycline against usual ecological bacterial flora of culture ponds of L. ROHITA. After applying pelleted feeds treated with oxytetracycline (OTC) at 2gm/kg the physicochemical parameters of water like temperature, pH, dissolved oxygen, alkalinity, ammonia and nitrate were recorded weekly: 28.99 to 31.09°C, 7.58-7.95 ppm, 5.36-5.86 mg/L, 86.31-111.99 mg/L, 0.20-0.30 mg/L and 0.11-0.17 mg/L, respectively. The total viable counts of bacteria were found 4.9±1.03×103- 5.75±1.0×103cfu/mL in pond water, 5.62±1.0×107- 6.60±1.02×107cfu/g in sediments, 6.77±1.0×106-7.57±1.0×106cfu/g in gills, 6.02±1.02×107-8.32±1.0×107cfu/g in gut of L. ROHITA in control ponds. After OTC treatment the total viable count of bacteria ranged from 3.1±1.19×103-3.1±1.20×103cfu/mL in water, 3.1±1.13×106-4.27±1.10×106cfu/g in sediment, 2.82±1.25×105- 3.09±1.19×105cfu/g gill, 2.69±1.12×106-4.68±1.12×106cfu/g in guts of L. ROHITA respectively, indicating reduction of overall bacterial load below 1 log in sediment, gills and guts of L. ROHITA significantly (P<0.005).
Keyword: Bacterial load, culture ponds, L. rohita, oxytetracycline.