ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2006
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2006 International Scholars Journals
Full Length Research Paper
High level production of the recombinant gag24 protein from HIV-1 IN Escherichia coli
Ramón E. Narciandi Díaz1*, José Motolongo Viquillon2, Luis Pérez Figueroa2.
1Department of Technology Transfer, 2Division of Vaccine HBsAg, Center for Genetic Engineering and Biotechnology, Ave 31 e/158-190, Playa, P.O.Box 6162, Havana 10600, Cuba. Web site: http//www.cigb.edu.cu.
Accepted 28 July, 2005
Abstract
The gag24 gene of the Human Immunodeficiency Virus type 1 (HIV-1) was expressed under the control of the tryptophan promoter in Escherichia coli. The effect of several parameters on the production of gag24 was studied. The expression level achieved (25%) depended on the host strain and the induction conditions. The developed fermentative process was scaled-up to 50 L, where 417 mg/L of volumetric production and 34 mg/L.h of productivity, were obtained.
Key words: Escherichia coli, gag24, HIV-1, scale-up.