ISSN 2167-0447
International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (2), pp. 001-006, February, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Optimized Rhizome Bud Culture for Accelerated Plantlet Regeneration in Alpinia purpurata
K.P. Kochuthressia1, S.John Britto1*, L. Joelri Michael Raj1, M.O.Jaseentha1 and S.R.Senthilkumar2.
1The Rapinat Herbarium and Centre for Molecular Systematics, St. Joseph’s College (Autonomous), Tiruchirappalli – 620 002, South India.
2Department of Plant Biology and Plant Biotechnology, St. Joseph’s College (Autonomous), Tiruchirappalli – 620 002, South India.
Accepted 11 November, 2025
A reproducible and efficient protocol is described for multiple shoot induction of Alpinia purpurata using rhizome bud explants. Murashige and Skoog medium supplemented with BA (3.0 mg/l) and Kn (2.0 mg/l) exhibited regeneration rate up to 6.4±0.32 shoots/explants. Spontaneous rooting of shoots occurred in the same concentration of cytokinins. The number of leaves (4.8±0.67) and profuse rooting (8.4±0.70) facilitated 100% of plant recovery on acclimatization. This protocol proves its utility for rapid propagation of A. purpurata to be exploited for pharmaceutical and commercial purposes.
Key words: Alpinia purpurata, micropropagation, rhizome bud culture.