ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2756-3855 Vol. 13 (2), pp. 001-006, February, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Exploring the Lack of Correlation Between PTEN Gene Promoter Methylation and Urocystic Tumor Development
Xiao-Feng Sun1, Zhong-Yi Sun2, Bo Pan1, Lan Li1 and Wei Shen1*
1Laboratory of Germ Cell Biology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao, 266109, China. 2Daping Hospital, Third Military Medical University, Chongqing, China.
Accepted 1 February, 2025
Tumor suppressor gene PTEN plays an important role in cell cycle. Disorder of PTEN protein can cause cell growth and division in an uncontrolled way, which can lead to the formation of tumors. It has been proven that epigenetic mechanisms, such as promoter hypermethylation, may account for inactivation of PTEN in a subset of tumors. PTEN promoter hypermethylation has been found to be involved in many kinds of cancers. Up to date, no report about the relationships between methylation of PTEN promoter region and bladder cancer has been found. To investigate the methylation pattern of PTEN gene transcriptional regulation region (TRR), bisulfite-specific (BSP) polymerase chain reaction (PCR)-based sequencing analysis was performed among 15 bladder cancer tissues and five normal bladder tissues. Analysis of PTEN gene TRR methylation showed that the methylation level in bladder cancer had no significant difference with that of normal (P = 0.4307, by unpaired Student’s t test).
Key words: PTEN, promoter methylation, bladder cancer.