ISSN 2756-3820
International Journal of Medical Sociology and Anthropology ISSN 2756-3820 Vol. 11 (4), pp. 001-005, April, 2022. © International Scholars Journals
Full Length Research Paper
Screening of phytochemical from ethnomedicinal plants in Malaysia for use against toxigenic Aspergillus flavus
Yazdani, D.1, 2, Zainal Abidin, M. A.2*, Tan, Y. H.2, Kamaruzaman, S.2 and Jaganath, I. B.3
1Department of Pharmacognosy and Pharmaceutic, Institute of Medicinal Plants, ACECR, Tehran, Iran.
2Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
3Biotechnology Research Centre, Malaysian Agricultural Research and Development Institute, Kuala Lumpur, Malaysia.
Accepted 9 March, 2022
Abstract
Medicinal plants have been used as traditional treatments for numerous diseases for thousands of years. Antifungal activity of phytochemicals extracted from nine plant species in Malaysia traditionally used for treatment of skin infections were tested against growth of aflatoxin-producing Aspergillus flavus isolated from rice grains. The results, evaluated by measuring the diameter of inhibitory zone in disk diffusion test, minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC), showed that chloroform soluble fractions of methanolic extract of Alpinia galanga (lengkuas) rhizomes could strongly inhibit the mycelia growth of A. flavus. The constituent of this phytochemical fraction was identified by HPLC-MS2 using ESI ionization technique. In total, 10 compounds were identified based on MS2 data and λmax at 280 nm. These results suggested the potential of A. galanga for used as botanical fungicide.
Key words: Alpinia galanga, antifungal, Aspergillus flavus, traditional medicine, phytochemicals, LC-MS2.