ISSN 2756-3847
International Journal of Plant Breeding and Genetics ISSN 2756-3847 Vol. 12 (5), pp. 001-007, May, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Evaluating Bioremediation Potential of Pleurotus tuber-regium Against Diverse Crude Oil Fractions in Soil Environments
Erute Magdalene Ogbo1* and John Ariyo Okhuoya2
1Department of Botany, Delta State University, Abraka, Nigeria
2Department of Botany, University of Benin, Benin City Nigeria
Accepted 19 March, 2025
The use of chemical fertilizer and animal manure on crude oil reduction during biodegradation with Pleurotus tuber-regium was investigated. The co-substrates and inocula types used for the investigation were shredded banana leaf blades and sawdust of Albezia and sclerotium and spawn of the fungus, respectively. The reduction of total petroleum hydrocarbons was higher in treatments with a combination of fertilizers and co-substrates. Degradation of total petroleum content was higher in poultry litter treatment than the NPK treatments. The degradation of aliphatics in substrates with co-substrates was higher than those without co-substrates. Reduction of aromatics was appreciable in all treatments with the least been ImageImage40% in banana leaf blades + contaminated soil and contaminated soil only substrates. Degradation of resins was higher in contaminated soils with co-substrates only than those with fertilizers + co-substrates. The reduction of asphaltenes was low and some substrates instead of a decrease, recorded increase in the asphaltic fraction. Phytoasssessment tests show that the addition of poultry litter and sawdust and banana leaf blades to fungal remediation restored the contaminated soil. The addition of NPK to crude oil contaminated soil in the presence of P. tuber-regium affected the soil negatively as it could not support plant growth anymore. P. tuber-regium is better able to remove hydrocarbons from soil with the aid of poultry litter and co-substrates.
Key words: Biodegradation, petroleum hydrocarbons, co-substrates, phyto-assessment, fertilizers.