ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 12 (8), pp. 001-004, August, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Multiplex PCR Analysis of Clostridium perfringens Isolates Associated with Necrotic Enteritis Outbreaks in Broiler Farms in Cairo
Effat, M. M1*; Abdallah, Y. A.2, Soheir, M. F.1, and Rady, M. M.3
1Microbiology and Immunology dept, National research centre, Giza, Egypt.
2Serum and Vaccine Research Institute, Abbassia, Egypt.
3Poultry Diseases dept, National Research centre, Giza, Egypt.
Accepted 25 July 2024
Abstract
During the winter of 2006, outbreaks of severe entritis affected many broiler farms in the AL-Fayoum governorate, south of Cairo, Egypt. To identify the causative agent(s), bacterial isolates (14) from the diseased chickens were characterized phenotypically and biochemically. All isolates exhibited characteristics of Clostridium perfringens. Subsequently, molecular typing of the bacterial isolates was performed by multiplex PCR using four sets of primers specific for the genes encoding the C. perfringens, and toxins, respectively. A single amplicon, corresponding in size to the alpha ( ) toxin-encoding gene (approximately 402 bp), was amplified from all the bacterial isolates. It was therefore concluded that only C. perfringens type A was responsible for the disease outbreaks.
Key words: Clostridium perfringens- necrotic entritis – broiler chicken – multiplex PCR.