ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 8 (3), pp. 001-004, March, 2014. © International Scholars Journals
Full Length Research Paper
Effect of acid hydrolysis of Garcinia kola (bitter kola) pulp waste on the production of CM-cellulase and β-glucosidase using Aspergillus niger
Caritas U. Okafoagu1 and Humphrey C. Nzelibe2*
1National Agency for Food and Drugs Administration and Control, Kaduna Area Laboratory, Kaduna, Nigeria.
2Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Accepted 21 September, 2013
Abstract
Effect of acid hydrolysis of bitter kola (Garcinia kola) pulp wastes on the production of CM-cellulase and β-glucosidase using Aspergillus niger was investigated. Hydrolysis for 3 h with 2.5 M sulphuric acid yielded highest fermentable sugar. Acid hydrolysis enhanced CM-cellulase and β-glucosidase levels by 500% at 96 h and 200% at 120 h, respectively. Acid hydrolysed G. kola pulp wastes is a better substrate for the production of CM-cellulase and β-glucosidase compared with maize and rice husks.
Key words: Bitter kola pulp wastes, CM-cellulase, β-glucosidase, Aspergillus niger, acid hydrolysis.