ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 9 (2), pp. 001-009, February, 2020. © International Scholars Journals
Full Length Research Paper
Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)
K. S. Sim1, A. M. Sri Nurestri 1* and A. W. Norhanom2
1Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
2Centre for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Accepted 16 September, 2019
Abstract
The total phenolic content of the extracts of Pereskia bleo were assessed by the Folin-Ciocalteau’s method while the antioxidant activities were determined by three different assays, namely scavenging activity on 1,1- diphenyl-2 -picrylhydrazyl (DPPH) radicals, reducing power assay and ß-carotene method. In the present study, the ethyl acetate extract exhibited the highest total phenolic content (40.12 mg of GAEs/g of extract) and showed the strongest antioxidant activity in the ß -carotene bleaching assay significantly. Whilst, the hexane extract showed significantly the highest antioxidant activity when determined by scavenging effect on DPPH radicals (EC50 210 µg/ml) and the reducing power assay.
Key words: Pereskia bleo, Cactaceae, antioxidant activity, phenolic content.