ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 5 (9), pp. 001-007, September, 2016. © International Scholars Journals
Full Length Research Paper
Prohibitive effect of lactoperoxidase system (LPS) on some pathogen fungi and bacteria
Melda Sisecioglu1, Ekrem Kirecci2, Murat Cankaya3, Hasan Ozdemir1, Ilhami Gulcin1,4* and Ali Atasever1
1Department of Chemistry, Faculty of Sciences, Ataturk University, 25240 Erzurum, Turkey.
2School of Health Service, Sutcu mam University, 46100-Kahramanmaras, Turkey.
3Department of Biology, Faculty of Sciences and Art, Erzincan University, 24100 Erzincan, Turkey.
4School of Health Services, Agri brahim Cecen University, TR-04100-Agri, Turkey.
Accepted 20 April, 2016
Abstract
Lactoperoxidase is a peroxidase enzyme found in milk. This enzyme has antimicrobial and antioxidant properties. It is fairly heat resistant and was widely used as an indicator of over pasteurization of milk. In the present study, bovine lactoperoxidase (LPO) was purified from skimmed milk using amberlite-CG-50-H+ resin, CM-sephadex-C-50 ion-exchange chromatography and sephadex-G-100 gel filtration chromatography. Enzyme activity was determined using 2, 2-azino-bis-diammonium salt as a chromogenic substrate at pH 6, and purification degree was controlled by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Antibacterial and antifungal activity of bovine lactoperoxidase was determined by disk diffusion method. Bovine LPO exhibited high antifungal and antibacterial activity in 100 mM thiocynate-100 mM hydrogen peroxide (H2O2) medium on some fungi (Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis and Saccharomyces boulardii) and bacteria (Citrobacter freundii, Escherichia coli, Streptococcus pneumoniae, Staphylococcus epidermidis and Staphylococcus intermedius), respectively. Antibacterial and antifungal activity of LPO system was compared to those of well known antibacterial and antifungal substances such as cefaclor, erythromycin, tetracycline and fluconazole. This study has investigated for the first time the antifungal and antibacterial effects of bovine LPO system on C. albicans, C. glabrata, C. krusei, C. parapsilosis, S. boulardii, S. pneumoniae, S. epidermidis and S. intermedius.
Key words: Milk, enzyme, lactoperoxidase, LPO, antibacterial, antifungal.