ISSN 2756-3316
African Journal of Medical Case Reports ISSN 2756-3316 Vol. 11 (1), pp. 001-009, January, 2023. © International Scholars Journals
Full Length Research Paper
Experimental models for balanced activity of oncogenes and tumor-suppressor genes in normal and malignant cells
Iskra Ventseslavova Sainova1*, Ilina Vavrek1, Velichka Pavlova1, Ivan Iliev1, Lilija Yossifova1, Elena Gardeva1, Elena Nikolova1, Teodora Daneva2, Roberto Nitsch3 and Anna Nitsch3
1Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
2Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Street, 1113 Sofia, Bulgaria.
3Institute of Molecular Biotechnology (IMBA) to Austrian Academy of Sciences, “Dr. Bohr” Street, 1030 Vienna, Austria.
Accepted 17 October, 2022
Abstract
Gene transfer in laboratory-cultivated mouse embryonic stem cells (mESCs) was made by appropriate recombinant DNA-constructs. Electrophorhetic profiles of genetic material from wild type (WT) on oncogene DCN1 and “knock-down” (KD) on it inbred lines of experimental mice differed not only on it, but also on the tumor-suppressor gene HACE1 between both categories of laboratory rodents. The results obtained were compared with previous data, received from malignant rat insulinoma RIN-5F cells, transfected by recombinant gene constructs with inserted copy of secretagogin gene, by their IN VITRO-co-cultivation with malignant cell precursors, derived from populations of non-transfected laboratory-cultivated mESCs in the presence of doxyciclin, probably by activation of tumor-suppressor genes of STAT-family. These data were confirmed by the differences noticed in the degree of myeloid differentiation of derived precursor cells in their IN VITRO-co-cultivation with containing additional copy of secretagogin gene Rin-5F malignant rat insulinoma cells, in comparison with the results, obtained in their laboratory co-cultivation with non-treated human cervical carcinoma Hela cells, as well as with derived normal mESCs, containing additional copy of the oncogene DCN1 as a result of their transfection with recombinant DNA-constructs. On the other hand, the derived normal cells with inserted additional copy of oncogene indicated safety, immunogenity, and they also indicated preserved normal cell characteristics.
Key words: Oncogenes, tumor-suppressor genes, myeloid cell precursors, recombinant gene constructs, cell transfection.