ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Screening and optimization of metal ions to enhance ethanol production using statistical experimental designs
Mary Anupama Palukurty1*, Naveen Kumar Telgana2, Hema Sundar Reddy Bora3, Shiva Naresh Mulampaka4
1,2,3Department of Biotechnology, ANITS, Sangivalasa, Visakhapatnam.
4Lecturer, Department of Biotechnology, MSRIT, MSR Nagar, Bangalore – 54.
Accepted 14 March, 2012
Abstract
Ethanol production using jaggery was enhanced in submerged fermentation when the effect of metal inducers was studied using the Plackett-Burman and Box-Behnken designs. Saccharomyces cerevisiae (NCIM 3288) was used as the fermenting organism. The Plackett-Burman design was used to initially screen seven of which the four elements were found to have significant effect on ethanol production. In the next stage, Box-Behnken design was used obtain concentrations of metal ion’s that may be supplemented to get maximum ethanol in during production process. It was observed that ethanol yield has increased to 94.8 from 75.4g/l when supplemented with the critical concentrations of salts provided by the model. These were as follows (g/l): FeSO4. 7H 2O 0.0036, MgSO4.7H2O 0.0033, MnCl2. 4H 2O 0.0017 and ZnSO4.7H 2O 0.0026, in the presence of 220 g/l of jaggery supplemented with (NH4)2SO4 2.612 g/l and KH2PO4 3.407 g/l, while the predicted concentration of ethanol as per the model is 95.35 g/l.
Key words: Jaggery, ethanol, Plackett-Burman design, Box-Behnken design, metal inducers.