ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 18 (3), pp. 001-010, March, 2024. https://internationalscholarsjournals.org/journal/ajmr/table-of-contents © International Scholars Journals
Full Length Research Paper
Chemical composition and antifungal activities of hydroethanolic extracts of cocoa (Theobroma cacao L.) pod waste, Ivory Coast
AKAFOU Ethien Felix1*, GOLY Kouassi Roselin Cyrille2, ATOBLA Koua3, SORO Yaya1 and ADIE Adjehi4
1Laboratoire des Procédés Industriels, de Synthèse et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix HOUPHOUET-BOIGNY de Yamoussoukro, BP 1093 Yamoussoukro, Côte d'Ivoire
2Department of Science and Technology, UFR of Medical Sciences, Alassane Ouattara University, Bouaké, 01 BPV 18 Bouaké 01, Côte d’Ivoire
3Department of Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
4Laboratory of Biotechnology and Food Microbiology, Department of Food Science and Technology, Nangui Abrogoua University, 02 BP 801, Abidjan 02, Côte d’Ivoire.
Received 23 December, 2023; Accepted12 February, 2024 and Published 6 April 2024
Abstract
Cocoa cultivation generates large quantities of cocoa pod shells, the management of which is a concern for producing countries. This study aims to valorize cocoa pod waste by determining the chemical composition and antifungal activities of their hydroethanolic extracts. Secondary metabolites were extracted from the dried powder of plant material by maceration in hydroalcoholic solvent. Their families were determined by phytochemical screening and certain contents determined by dosage. Antifungal activities were evaluated in solid and liquid media. Extraction yields were 14.10 ± 0.08 and 11.92 ± 1.81% respectively for green and yellow pod shells. Phytochemical screening revealed the presence of all the families sought in the yellow and green pod shells. Total polyphenol and flavonoid contents of yellow pods are higher than those of green pods with values of 35.50 ± 0.50 mg EAG/g and 65.33 ± 0.33 mg EQ/g of dry extract, respectively. However, condensed and total tannins are more abundant in green pods with contents of 16.40 ± 0.02 and 23.98 ± 0.02 mg EAT/g, respectively. Antifungal tests revealed that the four fungal strains tested were more sensitive to hydroethanol extract of yellow pod husks. Results obtained show that cocoa pod waste can be used in pharmaceutical field.
Key words: Cocoa pod waste, hydro-ethanolic extracts, secondary metabolites, antifungal activity.