ISSN 2756-3820
International Journal of Medical Sociology and Anthropology Vol. 6 (1), pp. 001-006, January, 2015. © International Scholars Journals
Full Length Research Paper
Leptin decreases the sensitivity of human colon cancer cell HT-29 to 5-flurouracil
Lei Shen1,2*, He-sheng Luo1,2, Jun Sun1,2 and Liang Zhao1,2
1Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
2238, Jiefang Road, Wuhan City, Hubei Province, Zip code: 430060, China.
Accepted 20 June, 2015
Abstract
To verify whether leptin attenuated the sensitivity of colon cancer cells to 5-flurouracil (5-FU). HT-29 cells were treated with 5-FU in the absence or presence of various concentrations of leptin. The cytoprotective effect of leptin was detected by MTT and flow cytometer. The expression of caspase-9 was measured by RT-PCR and Western blot analysis. Leptin attenuated the cytotoxic response of HT-29 cells to 5-FU by facilitating cells survival and antagonizing apoptosis. In addition, leptin decreased the expression of caspase-9 in HT-29 cells induced by 5- FU. Leptin treatment reduces the susceptibility of colon cancer cells to 5-FU by promoting cell survival. Attenuation of caspase-9 expression is one of the mechanisms underlying the cytoprotective role of leptin in HT-29 cells.
Key words: Leptin, apoptosis, caspase-9, drug resistance, 5-flurouracil.