ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 11 (4), pp. 001-007, April, 2022. © International Scholars Journals
Full Length Research Paper
Determination of gliclazide in a tablet dosage form in the presence of metformin hydrochloride by ion pair – reversed phase liquid chromatographic technique
B. Udaykumar Rao1* and Anna Pratima Nikalje2
1Maulana Azad Postgraduate and Research Centre, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, M.S. India.
2Y.B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, M.S. India.
Accepted 10 May, 2021
Abstract
This study describes the development and validation of an isocratic HPLC method for the stability of gliclazide assay in the presence of metformin hydrochloride (MET) in pharmaceutical dosage forms using ion pair - reversed phase liquid chromatographic technique. The ion pairing agent used in this study is tetrabutyl ammonium hydrogen sulphate (TBHS). The TBHS 0.030 molar solution in water was adjusted to pH 6.0 with 1 N sodium hydroxide solution and was used as buffer. The composition of the buffer and acetonitrile used was 40:60 (v/v) on reverse phased column, bonded with octadecyl silane, while the wave length used was 225 nm. As such, the drug gave a linear response (r2>0.999). The method was found to be selective, precise, accurate and robust, and can be used for quality control assay of the bulk and finished dosage form as a single dose in combination with metformin hydrochloride.
Key words: Ion pair, reversed phase, UV detection, gliclazide.