ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 15 (3), pp. 001-008, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Enhancing Lycopene Production in Tomato Roots through Genetic Transformation with Agrobacterium rhizogenes
Wahyu Widoretno1*, Ratna Perwitasari1, Estri Laras Arumingtyas1, Edi Priyo Utomo2, Tatik Wardiati3
1)Department of Biology, Faculty of Mathematics and Natural Science, University of Brawijaya Jl. Veteran Malang 65145
East Java Indonesia
2)Department of Chemistry, Faculty of Mathematics and Natural Science, University of Brawijaya Jl. Veteran Malang
65145 East Java Indonesia
3)Faculty of Agriculture, University of Brawijaya Jl Veteran Malang 65145 East Java Indonesia
Accepted November 25, 2024
Different cell density of Agrobacterium rhizogenes ATCC 15834 and inoculation time were tested to investigate the ability for transformation and production of 1ycopene in Lycopersicon esculentum Mill. Agrobacterim rhizogenes used in this study were able to produce hairy roots. The transformation frequency was 33-59%. Bacterial cell density and inoculation time didn’t affect on transformation frequency and root number per explants but affected growth and 1ycopene content of hairy root. The developed culture exhibited fast growth and high lateral branching on hormone-free liquid MS medium. Higher bacterial cell density OD and longer inoculation time decreased fresh weight of hairy roots. Cell density of Agrobacterium significantly affected 1ycopene content of hairy root while effect of inoculation length was non significant at 5% probability level. Cell density OD = 0.5 on all inoculation time produced 1ycopene content higher than that of cell density OD = 1.0. The highest fresh weight (1970 mg) and 1ycopene content (1.68 µg/g) was observed after 6 weeks of hairy roots culture produced by inoculation of Agrobacterium at cell density OD = 0.5 for 15 minutes. Lycopene content of root closely related to growth of hairy root.
Keywords: Agrobacterium rhizogenes, Lycopersicon esculentum, 1ycopene, hairy root, secondary metabolite, cell density, inoculation time.