ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2756-3855 Vol. 13 (4), pp. 001-005, April, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Impact of Pretreatment Methods and Environmental Conditions on Bladder-Senna Seed Germination
Zafer Olmez* and Askin Gokturk
Artvin Coruh University, Faculty of Forestry, 08000 Artvin, Turkey.
Accepted 11 November, 2024
This study was carried out to determine which pre-treatments should be preferred to overcome dormancy problems of Colutea armena seeds which were collected from three different provenances. Pre-treatments applied to the seeds were submersion in concentrated (98%) sulphuric acid for 30 min, floating in hot water (100°C) followed by continual cooling for 24 h in the same water, soaking in tap water (20 ± 1°C) for 24 h and cold stratification for 20, 40 and 60 days. The seeds were sown in polyethylene pots in the greenhouse and in seedbeds under open field conditions. The statistical approach was a randomized complete block design with three replications. Germinated seeds were observed periodically during 70 days to determine germination percentages and germination rates. The highest germination percentages (52.2, 56.7 and 60.5%) were obtained in seeds which were submersed in sulphuric acid for 30 min and sown in the greenhouse for all three provenances. The best germination rates for provenances of Köprüba ı and Derinköy were 14 and 16 days which were determined in seeds soaked in sulphuric acid for 30 min and sown in the greenhouse. On the other hand, the best germination rate (12 days) of provenance of Salkımlı was determined in seeds which were floated in hot water (100°C) followed by continual cooling for 24 h in the same water and sown under open field conditions.
Key words: Colutea armena, germination, seed, greenhouse, open field condition.