ISSN 2756-3693
African Journal of Biology ISSN 2167-0413 Vol. 2 (8), pp. 178-182, November, 2015. © International Scholars Journals
Full Length Research Paper
Analysis of selected Enterobacteriaceae for extended spectrum beta-lactamase (ESBL) production and virulence factors associated with fruit samples
Okome R. A*, Abdulahi M. Ali and Ahmadu E. Mohammed
Department of Biological Sciences, Microbiology unit, Fountain University, Osogbo, Osun State, Nigeria.
Accepted 15 September, 2015
E-mail: [email protected]
Virulence factors (such as serum resistance, cell surface hydrophobicity, haemolysin, and gelatinase production) and extended spectrum β lactamase were determined for the Gram negative bacilli isolated from ready-to-eat fruit samples comprising of Ananas comosus, Carica papaya, Citrus sinensis and Citrullus lanatus. The Enterobacteriaceae which include Klebsiella oxytoca (31.7%), Klebsiella pneumonia (22.0%), Proteus mirabilis (14.6%), Enterobacter gergoviae (13.4%), Proteus vulgaris (3.7%), Pantoea agglomerans (3.7%), Hafnia alvei (3.7%), Enterobacter aerogenes (2.4%), Citrobacter sakazaki (1.2%), Citrobacter freundii (1.2%), Salmonella arizonae (1.2%) and Serratia marcescens (1.2%) respectively were characterized using Microgen GNA ID kit. The haemolysin production analysis showed that 96.6% of Enterobacteriaceae produced cytolytic protein toxins (α-haemolysin), gelatinase (81.7%), serum resistance (87.9%), cell surface hydrophobicity (92.4%) and 95.8% were resistant to cefotaxime and ceftazidime which implies that such organisms are ESBL producers. The research showed that consumption of ready-to-eat fruits processed and sold by unlicensed vendors with poor education levels and untrained in food hygiene may increase the risk of food-borne diseases.
Key words: Serum resistance, haemolysin, extended spectrum β lactamase, fruit, enterobacteriaceae.