ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 15 (4), pp. 001-005, April, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Hormonal Regulation of In Vitro Flowering in Somatic Embryo Cultures of Kinnow Mandarin
B. Singh1, S. Sharma1, G. Rani1, G.S. Virk1, A.A. Zaidi2 and A. Nagpal1*
1Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India.
2Floriculture Division, Institute of Himalayan Bioresource Technology, C.S.I.R., Palampur 176 061 (H.P), India.
Accepted 28 Jauary, 2026
Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules dissected from unopened flower buds of this plant inoculated on MS medium supplemented with 2 mg/L kinetin (KN). In vitro flowering was induced in these cultures by using different concentrations of KN and sucrose as well as subjecting these cultures to different photoperiods. Maximum percentage (31.94%) of cultures producing flowers and maximum number (5.58) of flowers per culture was observed on MS medium supplemented with KN (2 mg/L) and sucrose 40 g/L at 12-h photoperiod.
Key words: Embryogenic callus, in vitro flowering, Kinnow (Citrus nobilis ´ C. deliciosa), ovule culture.