ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 11 (8), pp. 001-009, August, 2022. © International Scholars Journals
Full Length Research Paper
Biodegradable methoxy poly (ethylene glycol)-poly (lactide) nanoparticles for controlled delivery of dacarbazine: Preparation, characterization and anticancer activity evaluation
Bao-yue Ding1,2†, Wei Zhang1,3†, Xin Wu1, Xiang Wang1, Wei fan1, Shen Gao1*, Jing Gao4, Lu-lu Ma4, Xue-ying Ding4 and Qiang Hao5
1Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, Shanghai, PR China.
2Department of Pharmaceutics, Medical College of Jiaxing University, Jiaxing, PR China.
3Department of Pharmacy, No. 522 Hospital of PLA, Luoyang, Henan, PR China.
4Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
5Department of Radiology, Changhai Hospital, Second Military Medical University, Shanghai, PR China.
Accepted 10 May, 2022
Abstract
Dacarbazine (DTIC) loaded or blank methoxy poly (ethylene glycol)-poly (lactide) (MPEG-PLA) nanoparticles (NPs) were prepared by modified w/o/w double emulsion-solvent evaporation method through ultrasonic processor without any additional additives. The hemolytic test and cytotoxicity test of blank MPEG-PLA NPs demonstrated that the obtained drug delivery system is safety. Its particle size distribution, morphology, drug loading, drug release profile and anticancer activity in vitro were studied in detail. The small sized nanoparticles (NPs) with a particle size of 144.2±7.8 nm in diameter and drug encapsulation efficiency of 70.1±2.3% are easy to be dispersed in water and suitable for vascular administration. The drug release pattern was biphasic with a fast release rate followed by a slow one. The in vitro and in vivo study results demonstrated that compared with free DTIC, DTIC loaded MPEG-PLA NPs could induce more apoptosis of cancer cell and showed enhanced antitumor activity. The described DTIC loaded MPEG-PLA nanoparticle in this paper might be a novel potential formulation for metastatic melanoma therapy.
Key words: Malignant melanoma, dacarbazine, methoxy poly (ethylene glycol)-poly (lactide), double emulsion and solvent evaporation method, nanoparticles, anticancer activity.