ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene
Shree Prasad Thapa1, Chun Keun Lim2, Sam Kyu Kim2, Jun Mo Cho1, Jang Hyun Hur1 and Duck Hwan Park2*
1Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
2Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Accepted 2 August, 2017
Abstract
A rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.
Key words: Brenneria rubrifaciens, deep bark canker, PCR (polymerase chain reaction), rubrifacine.