ISSN 2756-3820
International Journal of Medical Sociology and Anthropology Vol. 9 (5), pp. 001-004, May, 2019. © International Scholars Journals
Full Length Research Paper
Pilot study of DNA extraction from archival unstained bone marrow slides: comparison of three rapid methods
Gari MA1*, Abuzenadah AM1, Chaudhary AG1, Al-Qahtani MH1, Al-Says FM2 and Dmanhouri G2
1Genomic Medicine Unit, King Fahd Medical Research Center; King Abdulaziz University, Jeddah 21412, Saudi Arabia.
2Hematology department, Faculty of Medicine, King Abdulaziz University, Jeddah 21412, Saudi Arabia.
Accepted 15 January, 2019
Abstract
DNA can be isolated from a variety of human sample sources including anti-coagulant whole blood, bloodstains, hairs, tissue samples and buccal epithelial cells. The purpose of this study was to compare yield and quality of DNA samples obtained with the use of three different methods. The ability of these procedures to provide DNA for polymerase chain reaction (PCR) amplification from archival unstained bone marrow slides was tested on 35 different patients’ slides. Boiling in distilled water (A), proteinase K/Tween 35 method coupled with simplified phenol/chloroform isoamyl alcohol protocol (B) and modified commercial nucleon extraction and purification protocol (C, Amersham Life Science) gave extraction efficiencies of 57, 74 and 100% respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival unstained bone marrow slides.
Key words: DNA, polymerase chain reaction, bone marrow slides, reagent kit.