ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 7 (7), pp. 001-007, July, 2018. © International Scholars Journals
Full Length Research Paper
Development and validation of stability indicating assay method of cetirizine hydrochloride by HPLC
Muhammad Imran Khan1, Ghulam Murtaza2*, Sher Awan3, Muhammad Iqbal1, Muhammad Khurram Waqas1, Akhtar Rasool4, Urooj Fatima1, Muhammad Hassham Hassan Bin Asad2, Anwar Kahlid5, Faisal Usman6, Qazi Najam-us -Saqib2, Shujaat Ali Khan4, Kalsoom Farzana6, Seema Mahmood7 and Izhar Hussain2
1Department of Pharmacy, University of Faisalabad, Faisalabad, Pakistan.
2Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
3Department of Pharmacy, University of Sargodha, Sargodha, Pakistan.
4Department of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
5Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
6Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
7Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Accepted 18 March, 2018
Abstract
A new simple and rapid high performance liquid chromatography (HPLC) method was developed for the determination of cetirizine hydrochloride (CTZ) in tablets using CLC-ODS reverse phase column (4.6 × 250 mm, 5 µm). Salicylic acid was used as internal standard. A mixture of methanol and water of 70:30 with pH 4 (adjusted with o-phosphoric acid) was used as mobile phase. The eluents were detected at 231 nm. The coefficient of determination of calibration curve for CTZ and salicylic acid in mobile phase were 0.9898 and 0.9925, respectively. The limit of detection for CTZ was 4 µg ml-1. The proposed method was successfully applied for the stability study of CTZ. The CTZ was found to be stable at accelerated condition of temperature and relative humidity after storage of six months. This method can be used for the routine quality control and dosage form assay of CTZ in pharmaceutical preparations.
Key words: High performance liquid chromatography, cetirizine hydrochloride, pharmaceutical preparations.