ISSN 2756-3820
International Journal of Medical Sociology and Anthropology Vol. 2 (3), pp. 001-004, March, 2012. © International Scholars Journals
Full Length Research Paper
Antioxidant and antibacterial activity of crude methanolic extract of Euphorbia prostrata collected from District Bannu (Pakistan)
Mushtaq Ahmad1, Abdus Saboor shah1, Rahmat Ali Khan1*, Farid Ullaha Khan1, Noor Aslam Khan1, Mir Sadiq Shah1 and Muhammad Rashid Khan2
1Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
2Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan.
Accepted 25 November, 2011
Abstract
Medicinal plants help in improving the human health that is primarily due to bioactive constituents such as flavonoids, alkaloids, saponins, cardiac glycosides and tannins. These compounds play important role in minimizing oxidative stress, cancer, impotency, cardiac dysfunction and microbial inhibition. Euphorbia prostrata (L) is a small prostrate, annual herb found all over the world including Pakistan. This study was designed to investigate the protective effects of E. prostrata against oxidative stress and antibacterial potential. Methanolic extract of E. prostrata were used in three different concentrations of 1, 3 and 5 mg/ml. The maximum inhibition was shown at highest concentration of 5 and at 3 mg/ml the moderate inhibition while at 1 mg/ml the minimum inhibition was shown. Similarly concentration of methnolic extract of E. prostrata exhibited excellent scavenging activity. The % scavenging of E. prostrata is directly proportional to the concentration. The order of % scavenging activity is 50 < 100 < 150 < 200 < 250 g/ml. Ascorbic acid is used as negative control
Key words: Antioxidant, antibacterial, Euphorbia prostrata, dimethyl sulfoxide (DMSO), 1,1-diphenyl-2-picrylhydrazyl (DPPH), ascorbic acid.