ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (2), pp. 001-008, February, 2017. © International Scholars Journals
Full Length Research Paper
Tissue distribution of intramuscularly and intratumouraly administered DNA plasmid harbouring apoptotic gene in mice
Ruzila I.1, Zeenathul N. A.2*, Nik-Mohd- Afizan N. A. R.1, Sheikh-Omar A. R.2, NorHidayah M.1 and Mohd-Azmi M. L.2
1Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
2Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Accepted 19 November 2016
Abstract
This study investigated the bio-distribution and persistence of plasmid DNA following intramuscular and intratumoural administration in a mice model. Validated quantitative method (real-time qPCR) was used to quantify plasmid distribution in the tissue samples collected at 15 min, 1 h, 24 h and 1 week after administration of 100 g (1.5 x 1013 copies) of naked plasmids. Plasmids remained in the circulating blood (3.6 ± 2.2 x 102copies/500 ng gDNA) and injected muscle (2.8 ± 1.1 x 105 copies/500ng gDNA) for up to 1 week post administration. Plasmids were also detected in opposite muscle, lung, kidney, spleen, lymph nodes, liver and heart only 1 h post-injection or more. After 2 weeks of treatment, plasmids were retained solely in the tumor mass. These results suggest the presently used recombinant DNA plasmid was benefited with its early transgene expression characteristic which could release the anti-cancerous effect within short dwelling time.
Key words: DNA vaccine, recombinant plasmid, real-time PCR, biodistribution.