ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 5 (1), pp. 001-008, January, 2016. © International Scholars Journals
Full Length Research Paper
Integron-plasmid mediated antibiotic resistance and virulence factors in clinical Salmonella enterica serovars in rural Western Kenya
David Miruka Onyango1*, Rose Kakai2, Waindi Eliud Nyandago1, B. Ghebremedhin3, W. Konig3 and B. Kong3
1Maseno University, Department of Zoology, Box 333, Maseno 40105, Kenya.
2Maseno University, School of Public Health and Community Development, Box 333, Maseno 40105, Kenya.
3Otto Von Guericke Universitat, Medical Faculty, Institute of Medical Microbiology Leipziger Str. 44; D- 39120 Magdeburg, Germany.
Accepted 24 October, 2015
Abstract
Integron-mediated antibiotic resistance is common among diverse Salmonella serovars. Phenotypic antibiotic resistance was correlated with the presence of intergron–mediated genes in 65 Salmonella typhimurium isolates using conventional microbiological methods and PCR gene amplification. The resistance genes detected in the 20 S. typhimurium isolates were mainly those identified as part of the chromosomal multiresistance cluster balPSE-1, blaTEM and aadA. The plasmid encoded sul 1/ 2 and aphA - 1, aac (6) -1 located on the chromosome were not observed, however strB and Grm genes were observed. Genes coding for PSE -1 was predominant in the isolates as compared to TEM. The two genes were distributed differently between the various resistance phenotypes as could be observed of variability in int gene. Pathogenicity also varied in relation to individual isolate antimicrobial mechanism profile. These findings demonstrate integron mechanism as a means of antibiotic resistance in the isolates.
Key words: Intergron–mediated, Salmonella typhimurium, multiresistance, pathogenicity.