ISSN 2756-3863
International Journal of Manures and Fertilizers ISSN 2756-3863 Vol. 11 (5), pp. 001-005, May, 2023. © International Scholars Journals
Full Length Research Paper
Environmental Listeria monocytogenes isolates from Ado-Ekiti, Nigeria and its antibiotic resistance pattern
David, O. M.* and Odeyemi, A. T.
Department of Microbiology, University of Ado-Ekiti, P.M.B. 5363, Ado-Ekiti, Nigeria.
Accepted 26 January, 2023
Abstract
Incidence of Listeria monocytogenes in cow manure, agricultural soil, and common vegetables sold in major markets in Ado-Ekiti, Nigeria was determined. Antibiotic resistant pattern of the isolates was examined by paper disk assay. A total of 196 environmental samples were cultured on a selective medium out of which 153 (78.1%) were positive for L. monocytogenes. Erythromycin was the most effective antibiotic against the isolates with the least resistance (28.1%) while chloramphenicol proved to be least effective with resistant of 52.29%. The multiple-antibiotic resistant pattern of the isolates showed augumentin/amoxicillin (33.3%), augumentin/erythromycin (24.18%), and cotrimoxazole/ chloramphenicol/amoxicillin (28.8%) to be most prominent. The least value was observed in cloxacilin/cotrimoxazole/gentamycin with 15.34%. The modal values of the Minimum Inhibitory Concen-trations (MICs) of the antibiotics to the isolates range between 4.0 and >16.0 µg/ml. cotrimoxazole and gentamicin recorded the highest MIC compared with other antibiotics.
Key words: Listeria monocytogenes, vegetable, cow dung, antibiotic resistance, environmental isolates.