ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 9 (12), pp. 001-005, December, 2020. © International Scholars Journals
Full Length Research Paper
Bioautographic determination of the antistaphylococcal components of the stem bark of Parkia biglobosa (JACQ) BENTH (MIMOSACEAE)
Udobi Chinweizu Ejikeme1*, Onaolapo Ademola Josiah2 and Abdulsalaam I. A2
1College of Science and Technology, Kaduna Polytechnic, Kaduna, Nigeria.
2Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria, Nigeria.
Accepted 09 October, 2020
Abstract
Bioautography, a scientific method which combines chromatography with bioassay was used to attempt to determine the bioactive components in the methanolic extract of the stem bark of Parkia biglobosa. Results obtained confirmed 4 compounds of different Rf values. Each of them showed activity against Staphylococcus confirming previous results reported and the method also showed the components were acting in synergy. Pseudomonas and Bacillus subtilis were not as susceptible as Staphylococcus to the components. P. biglobosa remains a hopeful source of compounds that can be effectively used against even resistant Staphylococcus.
Key words: Bioautography, methanolic, Parkia, Staphylococcus, synergy.