ISSN 2375-1231
African Journal of Crop Science ISSN: 2375-1231 Vol. 13 (4), pp. 001-008, April, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Evaluation of Pretreatment Efficiency in Durum Wheat Androgenesis: A Study on Green Haploid Production in Tunisian Cultivars
O. Slama Ayed1*, J. De Buyser2, E. Picard2, Y. Trifa1 and H. Slim Amara1
1Laboratoire d’amélioration génétique des Céréales, Institut National Agronomique de Tunisie, 43 Avenue Charles
Nicolle 1082 -Tunis- Mahrajène Tunisia.
2Laboratoire de Morphogenèse Végétale Expérimentale Haploïde, Bat. 360, Université Paris sud XI Orsay- 91405
Cedex, France.
Accepted 11 December, 2024
The production of double haploids through androgenesis is used by breeders to produce homozygous lines in a single generation. Androgenesis can be achieved by isolated microspore culture, which, however, allows the production of green haploids with a very low efficiency. In order to improve the overall green haploid regeneration in durum wheat, we compare the efficiency of eight different pretreatments. These pretreatments were: cold pre-treatment for 5 weeks, 0.3M mannitol for 12 days at 4°C; 0.3M mannitol for 7 days at 4°C; 0.7M mannitol for 5 days at 4°C; PEG4000 1.5% for 5 days at 4°C; PEG4000 1% for 15 days at 4°C; PEG4000 1% for 10 days at 4°C and control without pretreatment. The cold treatment for 5 weeks (4°C) is the most efficient and was used to test the androgenetic ability by microspore culture method of three Tunisian durum wheat cultivars (Razzek, Khiar and Azizi).This pre-treatment improves significantly the embryogenesis induction and green plants regeneration with green/albina ratio 2 for the cultivar “Khiar”. The PEG4000 1% for 10 days seems promising pre-treatment and this is important to perform new experiments to advance their response.
Key words: Isolated microspore culture, double haploid, durum wheat, pre-treatment.