ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (10), pp. 001-010, October, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Antiplasmodial in vitro and in vivo activities of crude extracts from Ozoroa obovata (Oliv) R.Fern. &A. Fern collected in Burkina Faso
Aboubakar Soma1,2, *, Mariam Ouedraogo1,2,3, Bonewendé Mohamed Belemlilga1, Abdel Aziz Mintoumba1,2, Farida Carelle Ariane Kaboré2,4, Tiami Jonas Coulibaly1,3, Abdoul Laurent Gilchrist Boly1, Harouna Soré2, Souleymane Compaoré1, Nicolas Ouedraogo2, Guillaume Sanou2, Noufou Ouedraogo1, Maminata Traoré-Coulibaly1 and Aristide Traoré1
1Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS/CNRST), Ouagadougou, Burkina Faso
2Laboratoire de Pharmacognosie, Centre National de Recherche et de Formation sur le Paludisme (CNRFP), Institut National de Santé Publique (INSP), Ouagadougou, Burkina Faso
3Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), École Doctorale Sciences de la Santé (ED2S), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso
4Laboratoire de Biologie Moléculaire de Génétique (LABIOGENE), Département de Biochimie et Microbiologie, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
Abstract
Received 22 September, 2025; Revised 02 October, 2025; Accepted 03 October, 2025; Published 21 October, 2025
Malaria remains the main health concern in sub-Saharan Africa. This study aimed to evaluate the in vitro and in vivo antiplasmodial activities of Ozoroa obovata extracts, a plant traditionally used to treat malaria in Burkina Faso. The macerate extracts were freeze-dried, and TLC analysis was used to analyze the phytochemical compounds. The in vitro antiplasmodial activity was evaluated using the Plasmodium lactate dehydrogenase (pLDH) method. The in vivo antimalarial activity of the extract was evaluated in mice infected with Plasmodium berghei using the Peters’ 4-day suppressive test. Thin-layer chromatography (TLC) analysis revealed the presence of flavonoids, sterols, triterpenes and tannins in both the aqueous and hydroethanolic extracts. The IC50 of the aqueous extracts were 58.59 ± 9.05μg/ml against the 3D7 and 56.14 ± 7.84 μg/ml against NF54 strains. The hydro-ethanolic extracts shown the IC50 of 161.10 ± 12.51μg/ml against the 3D7 and 76.28 ± 3.20μg/ml against NF54 strains. The percentages of reduction in parasitemia with the aqueous extract were 2.5%, 54.00%, and 92.00% at 100mg/kg, 250mg/kg, and 500mg/kg body weight, respectively. The hydroethanolic extract showed reductions of 7.00, 65.00, and 83.75% for the respective doses 100mg/kg, 250mg/kg and 500mg/kg body weight. These activities shown could justify the using of O.obovata in traditional medicine to treat malaria.
Keywords: Ozoroa obovata (Oliv) R.Fern. &A. Fern, Malaria, Antiplasmodial activity, Burkina Faso, In vitro, in vivo.