International Journal of Medical Sociology and Anthropology

International Journal of Medical Sociology and Anthropology Vol. 4 (5), pp. 001-006, May, 2015. © International Scholars Journals

Full Length Research Paper

Antioxidant activities and RP-HPLC identification of polyphenols in the acetone 80 extract of Salvadora persica

Emira Noumi¹*, Hafedh Hajlaoui¹, Najla Trabelsi³, Riadh Ksouri³, Amina Bakhrouf¹, Mejdi Snoussi^1,2

¹Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Monastir, Tunisie. Université de Monastir

²Laboratoire de Traitement des Eaux Usées, Centre de Recherches et des Technologies des Eaux (CERTE), Technopole de Borj-Cédria, BP 273- Soliman 8020.

³Laboratoire d’adaptation des plantes aux stress abiotiques, Centre de Biotechnologie, Technopole de Borj-Cédria (CBBC), BP 901, 2050 Hammam-Lif, Tunisie.

Accepted 13 June, 2014

Abstract

Salvadora persica L. is one of the oldest known oral hygiene tools with high therapeutic effects on cariogenic bacteria and periodontal pathogens due to its well documented chemical constituents. In this paper, and for the first time, we report the use of RP-HPLC technique to identify the polyphenols in the acetone 80 extract obtained from stems of S. persica. The antioxidant properties (total antioxidant capacity, total phenolic and tannin content, DPPH, reducing power, and -carotene bleaching tests) of the extract were also investigated. Our results showed the presence of eleven compounds: caffeic acid (5.82%), rutin trihydrate (2%), trans-cinnamic acid (1.58%), gallic acid (1.53%), resorcinol (1.33%), chlorogenic acid (1.14%), quercetin dihydrate (0.67%), naphtho-resorcinol (0.48%), catechine hydrate (0.24%), p-coumaric acid (0.11%), and 3.4 dihydroxyphenylacetic acid (0.01%). The diluted acetone extract showed weak antioxidant activities comparatively to the BHT (DPPH IC ₅₀ 75 µg/ml; reducing power EC₅₀, 1940 mgml^-1 and IC₅₀= 460 µg/ml for the -carotene bleaching test) . The total antioxidant activity was estimated at 0.528 mg GAE.g ^-1 DW, the total phenolic content was 0.443 mg GAE.g^-1 DW and the total condensed tannin were 0.39 mg EC.g ^-1 DW. These results confirm the wide range of the biological activities of S. persica and reinforce the use of this plant of a natural source of antioxidant compounds with benefic properties for Human health.

Key words: Salvadora persica, RP-HPLC, polyphenols, antioxidant activities.