ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 3 (5), pp. 001-005, May, 2012. © International Scholars Journals
Full Length Research paper
Preliminary phytochemical, analgesic and anti-inflammatory studies of the methanol extract of Anisopus mannii (N.E.Br) (Asclepiadaceae) in rodents
A. M. Musa1*, A. B. Aliyu2, A. H. Yaro3, M. G. Magaji4, H. S. Hassan1 and M. I. Abdullahi1
1Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria, Nigeria.
2Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.
3Department of Pharmacology, Bayero University, Kano, Nigeria.
4Department of Pharmacology and Clinical Pharmacy, Ahmadu Bello University, Zaria, Nigeria.
Accepted 10 January, 2012
Abstract
The methanol extract of the aerial parts of Anisopus mannii was evaluated for its analgesic and anti-inflammatory activities. The analgesic effect was studied using acetic acid-induced abdominal constriction test in mice, while the anti-inflammatory effect was investigated using carrageenan induced paw oedema in rats. The results of the study showed that the extract (40 mg/Kg) exhibited significant (P < 0.01) analgesic effect. It also exhibited significant (P < 0.01) anti-inflammatory effect at a dose of 20 mg/kg. Phytochemical screening of the extract revealed the presence of alkaloids, flavonoids, saponins, steroids and tannins. The extract was found to have an intraperitoneal LD50 of 282.8 mg/kg in mice. The results showed that the extract contains some pharmacologically active principles and lend pharmacological credence to the ethnomedical use of the plant in the management of pain and inflammatory conditions.
Key words: Anisopus mannii, phytochemical screening, analgesic activity, anti-inflammatory activity, toxicity.