ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 9 (1), pp. 001-007, January, 2020. © International Scholars Journals
Full Length Research Paper
Plasmid DNA analysis and conjugative study of antibiotic resistant Salmonella typhi and paratyphi to ten selected antibiotics in Zaria, Nigeria
Adeshina Gbonjubola Olusesan*, Osuagwu Nonyelum, Okeke Claire-Lorentz Ebere and Ehinmidu Joseph Olorunmola
Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Accepted 16 July, 2019
Abstract
The antibiotic susceptibility of Salmonella typhi and Salmonella paratyphi isolates to ten selected antibiotics, plasmid DNA profiles and conjugative ability in Zaria, Nigeria were investigated. Blood samples collected from presumptive typhoid fever patients in the different locations were cultured for Salmonella species and identified by standard procedures. Susceptibility testing and minimum inhibitory concentration determination were performed using appropriate microbiological methods. Conjugative experiment was carried out with multiple antibiotics resistant isolates of Salmonella species. The resistant test bacteria strains were subjected to DNA isolation and characterization. Susceptibility study of test bacteria to ten selected antibiotics showed high percentage resistance to nine antibiotics such as Ampicillin, Amoxicillin, Augumentin, Chloramphenicol, Co -trimoxazole, gentamicin, Nitrofurantoin and Tetracycline (40 - 100%). Antibiotics resistance profile of S. paratyphi isolates were observed to be considerably higher than S. typhi isolates. The result of conjugation studies of multiple antibiotics resistant Salmonella species with Ofloxacin sensitive Escherichia coli ATCC 25722 showed that eight of the 18 Ofloxacin resistant Salmonella species isolates possess transferable resistant trait. The multiple antibiotics resistant (MAR) of test bacteria showed transferable plasmid sizes of 23.13 and 0.145 kb conferring resistance to the ten selected antibiotics. The findings from the plasmid analysis showed that the antibiotic sensitive S. typhi strains could acquire the R-plasmid from any resistant enteric bacteria such as E. coli, to undergo a suitable adaptation for survival in the changing antibiotic environment.
Key words: Plasmid, antibiotics, resistance, Salmonella species.