ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 7 (12), pp. 001-008, December, 2018. © International Scholars Journals
Full Length Research Paper
Rapid and simultaneous determination of aspirin and dipyridamole in pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) method
K. Prakash1, Rama Rao Kalakuntla2,3* and Jayapal Reddy Sama4
1SN Vanitha Mahavidyalaya Pharmacy College for Women, Hyderabad, Andhra Pradesh, India.
2Sree Dattha Institute of Pharmacy, Sheriguda, Hyderabad, Andhra Pradesh, India.
3Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
4Department of Animal Sciences, School of Life Sciences, University of Hyderabad, India.
Accepted 08 August, 2018
Abstract
The combination of Dipyridamole and Aspirin and is widely used to reduce thrombosis in patients with thrombotic diseases. A rapid, simple, precise and cost effective and reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed and validated for the simultaneous determination of Aspirin and Dipyridamole in pharmaceutical formulations. Separation of both Aspirin and Dipyridamole was achieved within 5 min with required resolution, accuracy and precision thus enabling the utility of the method for routine analysis. Chromatographic separation was achieved on a waters symmetry C18 3.5 µm, 50 x 4.6 mm using a mobile phase consisting of 0.1% ortho phosphoric acid and acetonitrile in the ratio of 75:25 at a flow rate of 1.0 ml per minute. The detection was made at 227 nm and the retention time of Aspirin and Dipyridamole were1.5 and 2.8 minutes respectively. The method was found linear over the range of 4 to 80 µg/ml for Dipyridamole and 0.5 to 10 µg/ml for Aspirin.
Key words: Aspirin, Dipyridamole, high performance liquid chromatography.