International Journal of Medical Sociology and Anthropology

International Journal of Medical Sociology and Anthropology Vol. 1 (2), pp. 001-009, December, 2011. © International Scholars Journals

Full Length Research Paper

Modeling a murine model of immunoglobulin-E (IgE)-mediated qingkailing injection anaphylaxis

Guoping Liao, Wenshi Li, Shuai He and Zhongyi Zhang*

Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, P. R. China.

Accepted 19 July, 2011

Abstract

Qingkailing injection (QKLI) is a classic compound traditional Chinese medical injection (TCMI). It can cause anaphylatic shock and death in clinic. Modeling a murine model of qingkailing injection anaphylaxis appears to be essential. In this study, for the first time, we used several strategies to prepare cationic bovine serum albumin (cBSA) and QKLI-cBSA conjugate. The native bovine serum albumin (nBSA), cBSA and QKLI-cBSA conjugate were separated by cationic exchange method and also identified by ultraviolet-visible (UV-Vis) and fourier transform infrared (FT-IR) spectrometry. Balb/c mice were then treated with subcutaneous injection of QKLI-cBSA plus alum. Serum was analyzed for total IgE and histamine levels by enzyme-linked immunosorbent assays (ELISAs), respectively. Lung reactions were evaluated by analyzing lung pathologic changes. QKLI-cBSA group mice were significantly and successfully sensitized. QKLI-cBSA conjugate significantly increased total IgE and Histamine in Balb/c mice serum. Moreover, histological examination of QKLI-cBSA conjugate treated mice showed acute injury with pulmonary alveoli and injury of trachea, as well as neutrophilic cell infiltration. In conclusion, we successfully established a murine model of immunoglobulin-E (IgE)-mediated qingkailing injection anaphylaxis. This model should provide a useful tool for detecting allergens in QKLI and for exploring new allergens-detecting approaches of other TCMIs.

Key words: Qingkailing injection, murine, immunoglobulin-E (IgE)-mediated anaphylaxis, Qingkailing injection (QKLI) - cationic bovine serum albumin (cBSA) conjugate.