Global Journal of Endocrinology and Diabetes

Global Journal of Endocrinology and Diabetes Vol. 9 (2), pp. 001-008, February, 2024. © International Scholars Journals

Full Length Research Paper

Correlation between serotonin levels in plasma and platelets in adolescents with type 1 diabetes

Gabriel Manjarrez-Gutiérrez^1,2*, Rocío Herrera-Márquez³, Teresa Neri-Gómez¹, Jorge Hernández-Rodríguez⁴

¹Laboratory of Molecular Pathology, Cardiology Hospital, National Medical Center (CMN-SXXI), Mexican Institute of

Social Security (IMSS), Mexico City, Mexico

²Medical Research Unit in Neurological Diseases, Specialties Hospital, CMN-SXXI, IMSS, México, DF, México

³Pediatric Hospital, CMN-SXXI, IMSS, Mexico City, Mexico

⁴Laboratory of Neurontogeny, Department of Physiology, Biophysics and Neurosciences, Center of Research and

Advanced Studies (CINVESTAV) IPN, Mexico City, Mexico.

Accepted 18 September, 2023

Abstract

The aim of this study was to determine, in adolescents with type 1 diabetes, 5-hydroxytryptamine (5-HT, serotonin) levels in plasma and platelets, 5-HT transporter (SERT), platelet 5-HT_2A receptor and transglutaminase 2. We performed a cross-sectional studying two groups: 1) adolescents with type 1 diabetes and 2) controls. Three milliliters of blood was obtained from all patients to determine 5-HT levels in plasma and platelets. Furthermore, platelets were obtained to evaluate SERT expression, 5-HT_2A receptor and transglutaminase 2 using Western blot. Dot blot was used for 5-HT. Glucose, glycated hemoglobin, albumin, triglycerides, cholesterol, hemoglobin, mean platelet volume and platelet count were also measured. Results were analyzed using Student t and Mann-Whitney U tests; P < 0.05 was accepted as statistically significant. Adolescents with type 1 diabetes had an elevation of plasma glucose and glycated hemoglobin (P < 0.001) as well as an increase the 5-HT levels in plasma and platelets along with a decreased expression of SERT, 5-HT_2A and increased transglutaminase 2 in platelets compared to controls (P < 0.05). Increased the 5-HT levels in plasma and platelets, together with the decreased expression of SERT and 5-HT_2A receptors and increase in transglutaminase 2 platelets, may have an important role in the prothrombotic state frequently presented by patients with diabetes.

Keywords: Serotonin, type 1 diabetes mellitus, platelets, SERT, 5-HT_2A receptor, transglutaminase 2