African Journal of Chemistry ISSN 4391-3199 Vol. 9 (5), pp. 001-006, May, 2022. © International Scholars Journals

Full Length Research Paper

Efficacy test of Melaleuca leucadendra and Callistemon viminalis essential oils on in vitro control of a strain of Aspergillus flavus isolated from peanut seeds in Senegal

Youssoupha DIOP^1*, Abdoulaye FAYE², Diégane SARR¹, Atanasse COLY ³, Saliou NGOM ⁴

¹Alioune Diop University of Bambey, UFR SATIC, EMEPA, Website: www.uadb.edu.sn, BP 30 Bambey, Senegal

²Directorate of Plant Protection (DPV), Laboratory of Phytopathology; BP: 20054 Thiaroye, Senegal

³Cheikh Anta Diop University of Dakar (UCAD)/Faculty of Science and Technology/Laboratory of Phytochemistry and Analysis (LPA), BP: 5005 Dakar-Fann.

⁴Senegalese Institute of Agricultural Research (ISRA), Routes des hydrocarbures BP : 3120 Dakar.

Accepted January 16, 2022 

Abstract

This study consists of an efficacy test of essential oils of two plant species of the Myrtacae family (Melaleuca leucadendra and Callistemon viminalis) on the in vitro control of a strain of Aspergillus flavus potentially aflatoxinogen isolated from peanut seeds in Senegal. To do this, different doses (750, 500, 250 and 100 ppm) of each oil were associated with the culture medium Czapek Yeast Extract Agar (CYA) and the development of the strain on the different culture media was followed for 7 days, after which the mycelial growth (mean colony diameter) and the sporulation density (average number of conidia per mm2) were evaluated in the 4 repetitions of each treatment. The results showed that C. viminalis essential oil at the higher dose of 750 ppm (T5) was found to be more effective, just after Mancozeb at 500 ppm (T9) used as the reference control, with no significant difference. This T5 was closely followed by the T6 (Callistemon 500 ppm), T1 (Melaleuca 750 ppm) and T2 (Melaleuca 500 ppm) treatments on the inhibition of fungus growth and reproduction. 

Key words: Essential oils, Callistemon viminalis, Melaleuca lecadendra, Aspergillus flavus, Senegal.