African Journal of Internal Medicine

African Journal of Internal Medicine ISSN 2326-7283 Vol. 8 (11), pp. 001-007, November, 2020. © International Scholars Journals

Full Length Research Paper

Diethylnitrosamine-induced hepatocarcinogenesis in rats: possible chemoprevention by blueberries

*Nermin A.H. Sadik, Shohda A. EL-Maraghy, Manal F. Ismail

Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Accepted 14 April, 2020

Abstract

Nitrosamine compounds are known hepatic carcinogens. This study was designed to study the efficacy of dietary supplementation with blueberries (BB) on diethylnitrosamine (DEN)-initiated hepatocarcinogenesis in male wistar rats. Rats were divided into three groups. The first group served as normal control group, the second group received DEN at a dose of 10 mg/kg body weight five times a week for 15 weeks. The third one received DEN as in DEN-treated group simultaneously with 4% BB-supplemented diet. The results showed that BB caused significant decrease in the elevated serum levels of -fetoprotein (AFP), homocysteine (Hcy) along with levels of glutathione(GSH), deoxyribonucleic acid (DNA), ribonucleic acid (RNA)and activity of glutathione reductase (GR) in liver. Normalization of elevated 2-macroglobulin ( 2M) and total antioxidant capacity (TAC) levels in serum, hepatic glutathione-S-transferase (GST), glutathione peroxidase (GPx) activities and liver weight was achieved whereas body weight was significantly decreased. Moreover, no significant change was observed in elevated relative liver weight, hepatic glucose-6-P-dehydrogenase (G6PD), lactate dehydrogenase (LDH) along with serum aminotransferases, alkaline phosphatase (ALP) and - glutamyltransferase ( -GT) activities. Significant increase in reduced hepatic activity of xanthine oxidase (XO) was achieved and histopathological damage was minimized in BB-treated group. It is suggested that BB suppress DEN- induced hepatocarcinogenesis and could be developed as a promising chemopreventive natural supplement for liver cancer.

Key words: Blueberries, diethylnitrosamine, hepatocarcinogenesis, 2-macroglobulin, -fetoprotein, homocysteine, glutathione, rats.