African Journal of Parasitology Research

African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (1), pp. 001-006, January, 2021. © International Scholars Journals

Full Length Research Paper

Detection and characterization of multidrug resistance and extended-spectrum-beta-lactamase-producing (ESBLS) Pseudomonas aeruginosa isolates in teaching hospital

Zahra Tavajjohi1, Rezvan Moniri2* and Ahmad Khorshidi2

1Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.

2Kashan University of Medical Sciences, Kashan, Iran.

Accepted 10 November, 2020

Abstract

Pseudomonas aeruginosa is the most common pathogen causing nosocomial infections. The objective of this study was to investigate the extended-spectrum-beta-lactamase (ESBLs) producing and multidrug resistance of hospital isolates of P. aeruginosa and to determine the presence of several resistance genes. A total of 86 isolates of P. aeruginosa were collected from teaching hospital in Kashan, Iran. Susceptibility to eight antimicrobial agents was performed by disk diffusion method. ESBL-phenotypic detection was carried out by double-disk synergy test; and the presence of the genes encoding of bla(TEM), bla(SHV), bla(CTX-M), bla(OXA) and bla(GES)-like genes was studied by polymerase chain reaction. The prevalence of ESBLs was 8.1%. The presence of genes encoding ESBLs was confirmed in seven isolates, comprising seven bla(GES-2), one bla(SHV-1), one bla(SHV-5) and one bla(CTX-M-1) genes. P. aeruginosa demonstrated the highest resistance rate to piperacillin (38.4%), 67.5% of isolates were sensitive to imipenem whereas 32.5% were MDR (resistant to three or more classes of antibiotics). A multidrug-resistant (MDR) phenotype occurred frequently in P. aeruginosa. bla(GES-2), which compromises the efficacy of imipenem detected in all of seven ESBL-producing P. aeruginosa strains. Proper infection control practices and barriers are essential to prevent spreading and outbreaks of ESBL-producing and MDR P. aeruginosa in our teaching hospital.

Key words: Pseudomonas aeruginosa, extended-spectrum-beta-lactamase, multidrug-resistant.