African Journal of Agricultural Economics and Rural Development

African Journal of Agricultural Economics and Rural Development ISSN 2375-0693 Vol. 10 (3), pp. 001-007, March, 2022. © International Scholars Journals

Full Length Research Paper 

RAPD and SSR analysis of wild oats (Avena species) from North West frontier Province of Pakistan

Zahid Hanif1, Zahoor A. Swati1, Imtiaz Khan2*, Gul Hassan2, K. B. Marwat2, sabz Ali3 and M. Ishfaq Khan2

1Institute of Biotechnology and Genetic Engineering, NWFP, Agricultural University Peshawar-Pakistan.

2Department of weed science, NWFP Agricultural University Peshawar-Pakistan.

3Plant breeding group, Plant research international, Wageningen University, Netherlands.

Accepted 26 November, 2021

Abstract

Genetic diversity of 10 biotypes of wild oats (Avena species) which differ in certain morphological and physiological characters were analyzed using 9 Randomly Amplified Polymorphic DNA (RAPD) and 2 Simple Sequence Repeat (SSR) primer sets. RAPD and SSR primers revealed different levels of genetic polymorphisms among the various biotypes. Range of genetic distances estimated by using individual primers was 0 – 100 % but on an average genetic distances were in the range of 30 - 65% indicating that these biotypes were genetically very diverse and possess a high degree of polymorphism.

Key words: Wild oats, DNA, Polymorphism, RAPD, SSR.