International Journal of Bacteriology and Mycology

International Journal of Bacteriology and Mycology ISSN 2756-3669 Vol. 8 (1), pp. 001-013, January, 2020. © International Scholars Journals

Full Length Research Paper

Biochemical and textural properties of frozen stored (-22˚C) gilthead seabream (Sparus aurata) fillets

Maria Makri

Aquaculture and Fisheries Department, Technological Educational Institute of Messolonghi, Nea Ktiria 30200, Messolonghi, Greece. E-mail: mmakri@teimes.gr. Tel: +302631058204. Fax: +302631058287.

Accepted 21 December, 2019

Abstract

Skinned, vacuum packed post-rigor gilthead seabream (Sparus aurata) fillets were stored frozen at - 22°C for up to 340 days. Sampling was carried out on fresh fillets at days 34, 91, 183, 266 and 340 of frozen storage. Tests related to muscle integrity (activity of -glucosidase and the protein content of centrifugal tissue fluids), myofibrillar protein denaturation (Ca2+- and Mg2+-ATPase activities in actomyosin extracts) and lipid degradation products (free fatty acids, peroxide values and thiobarbituric reactive substances) showed that storage time affected the integrity of muscles, and caused structural changes to myosin (or ‘actomyosin’) and hydrolysis and oxidation of lipids. A slight decrease in salt soluble proteins was observed after 266 days of frozen storage suggesting that storage time hardly affected the formation of aggregates. The water holding capacity of the stored frozen fillets decreased with storage time and was associated with the damage in muscle structures (protein content in centrifugal tissue fluids), denaturation of myofibrillar proteins and lipid degradation products (free fatty acids and peroxide value). The firmness and toughness of the frozen fillets, as measured by the Warner-Bratzler shear knife, increased slightly with storage time. The changes in toughness were associated with the state of myofibrillar proteins and changes in water holding capacity of the stored frozen fillets.

Key words: Gilthead seabream, fillets, frozen storage, quality.