International Journal of Urology and Nephrology ISSN 2756-3855 Vol. 10 (4), pp. 001-007, April, 2022. © International Scholars Journals
Full Length Research Paper
The consequences of the effects of the chemotherapeutic drug (vincristine) in organs and the influence on the bioavailability of two radio-biocomplexes used for bone evaluations in balb/c female mice
Deise Mara Machado de Mattos1,2,7*, Diré, G. F.1,2,4,5, Lima, R. C.1, Almeida, A. C. C.1, Bellucio D.4, Azevedo, C. S. S.1, Azevedo, S. S. S.1, Nascimento, S. F.1, Borba, H. R.4, Carvalho, J.J.5 and Bernardo Filho, M2,6
1Universidade Estácio de Sá. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brazil.
2Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofísica e Biometria, Rio de Janeiro, RJ, Brazil.
3Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Histologia, Rio de Janeiro, RJ, Brazil.
4Universidade Federal Rural do Rio de Janeiro, Instituto de Biologia, Departamento de Biologia Animal, Laboratório de Atividade Anti-helmíntica de Plantas. Seropédica, Rio de Janeiro, RJ, Brazil.
5Centro Universitário da Zona Oeste- UEZO, Avenida Manuel Caldeira de Alvarenga, 1203. Campo Grande, RJ 23070- 200, Brazil.
6Instituto Nacional do Câncer, Coordenadoria de Pesquisa, Praça Cruz Vermelha, Rio de Janeiro, RJ, Brazil.
7Universidade Estácio de Sá, Instituto das Ciências da Saúde, Centro de Pesquisas da Saúde - Campus R9, Rua André Rocha, 838, Taquara - Jacarepaguá, Rio de Janeiro, RJ, Brasil, 22740-361.
Accepted 16 October, 2021
The development of animal model to evaluate the toxicological action of compounds used as pharmaceutical drugs is desired. The model described in this work is based on the capability of drugs to alter the bioavailability of radiopharmaceuticals (radiobiocomplexes) labeled with technetium-99 m (99mTc). There are evidences that the bioavailability or the pharmacokinetic of radiobiocomplexes can be modified by some factors, as drugs, due to their toxicological action in specific organs. Vincristine is a natural product that has been utilized in oncology. The vincristine effect on the bioavailability of the radiobiocomplexes 99mTc-methylenediphosphonic acid ( 99mTc-MDP) and 99mTc-pyrophosphate (99mTc-PYP) in Balb/c female mice was evaluated. The fragments of kidney were processed to light microscopy and transmission electron microscopy. The aim of this work was to study at structural and ultrastructural levels the alterations caused by vincristine in organs. One hour after the last dose of vincristine, 99mTc-PYP or 99mTc-MDP was injected, the animals were sacrificed and the percentage of radioactivity (%ATI) was determined in the isolated organs. Concerning 99mTc-PYP, the %ATI (i) decreased in spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, lung, liver, pancreas, stomach, heart and brain and (ii) increased in bone and thyroid. Concerning 99mTc-MDP, the %ATI (iii) decreased in spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, liver, pancreas, stomach, heart, brain, bone, ovary and uterus. In conclusion, the toxic effect of vincristine in determined organs could be responsible for the alteration of the uptake of the studied radiobiocomplexes.
Key words: Radiobiocomplexes, vincristine, drug interaction, nuclear medicine, oncology.