International Journal of Public Health and Epidemiology

International Journal of Public Health and Epidemiology Vol. 1 (3), pp. 040-041, December, 2012. © International Scholars Journals

Full Length Research Paper

Standard serological tests for diagnosis of bovine brucellosis in Mathura district of Western Uttar Pradesh, India

Indu Sharma¹* and B. Bist²

¹Department of Microbiology, Assam University, Silchar-788011, India.

²Department of Veterinary Public Health, U.P. Pt. Deen Dayal Upadhaya, Pashu Chiktsa, Vigyan Vishwa Vidhalaya, Ewam Go-Anusandhan, Sansthan, Mathura-281001, India.

*Corresponding author. E-mail: [email protected]  

Received 12 October, 2012; Accepted 26 November, 2012

Abstract

A total of 56 serum samples from suspected cattles and 5 from brucellosis-free non- vaccinated cattle herd were collected from various livestock farms in Mathura district of Western Uttar Pradesh, of which 16 serum samples found to be positive were subjected to all the three serological tests that is Rose Bengal plate test (RBPT), Standard tube agglutination test (STAT) and Dot- enzyme linked immunosorbant assay (ELISA) respectively. The isolation of Brucella abortus from cervical swab samples was vital in the confirmation of Brucella infection and epidemiological evaluation of the herd. High titer of 1:320 and 1:640 was observed in the present investigation. Two (12.5%) isolates out of 16 samples were positive by STAT and all the 6 RBPT positive samples (100%) exhibited negative results by STAT. However, it was observed that all the 16 samples (100%) which earlier revealed to be negative for RBPT and STAT exhibited positive results with Dot- ELISA. In the present study though Dot-ELISA proved to be the most sensitive of the 3 tests used, it can, however be suggested that a combination of RBPT and Dot ELISA should be used, especially in case of  those samples that are found to be negative by either RBPT or STAT used alone or in combination.

Key words: Brucellosis, Rose Bengal plate test (RBPT), Standard tube agglutination test (STAT), Dot enzyme linked immunosorbant assay (ELISA).