ISSN 2375-1096
International Journal of Agroforestry and Silviculture ISSN 2375-1096 Vol. 7 (5), pp. 001-006, May, 2019. © International Scholars Journals
Full Length Research Paper
In vitro propagation of Ceropegia juncea Roxb
Krishnareddy P. V.1, Karuppusamy S.2 and Pullaiah T.1*
1Department of Botany, Sri Krishnadevaraya University, Anantapur‐515 003, Andhra Pradesh, India.
2Department of Botany, The Madura College, Madurai, Tamil Nadu, India.
Accepted 21 January, 2019
Abstract
The present study was conducted to establish a protocol for in vitro propagation of an endemic medicinal plant Ceropegia juncea (Asclepiadaceae) from one month old aseptic seedling nodal explants. The highest shoot multiplication rate of 20.65 ± 0.20 shoots/explant was achieved on Murashige and Skoog medium supplemented with BAP 8.87 mM + TDZ 4.54 mM. Excised shoots were rooted on half strength MS medium with IBA 4.90 mM + NAA 1.27 mM. 78% of the rooted shoots survived in the field.
Key words: Ceropegia juncea, in vitro propagation, endemic medicinal plant, nodal explants, multiple shoots.