ISSN 2756-3839
International Journal of Nematology and Entomology ISSN 2756-3839 Vol. 12 (2), pp. 001-006, February, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Evaluation of the In Vitro Anthelmintic Efficacy of Anogeissus leiocarpus and Daniellia oliveri Against Haemonchus contortus
Kabore Adama1*, Belem A. M. Gaston2, Tamboura Hamidou H.1, Traore Amadou1 and Sawadogo Laya3
1Institut de l’Environnement et de Recherches Agricoles (INERA)/Département Productions Animales (DPA), 04 BP
8645 Ouagadougou/Burkina Faso.
2Institut du Développement Rural (IDR)/Université Polytechnique de Bobo-Dioulasso, 01 BP 3770 Ouagadougou 01,
Burkina Faso.
3Université de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Accepted 12 October, 2024
A study was conducted to evaluate Anogeissus leiocarpus leaf and Daniellia oliveri stem barks as effective remedy for gastrointestinal parasites. The anthelmintic activity of these extracts on eggs, first stage larvae and adults of Haemonchus contortus was examined by in vitro tests. The extracts were prepared to obtain six increasing concentrations. This was done with Phosphate Buffered Saline (PBS) for egg hatch, embryonated egg assays (75, 150, 300, 600, 1 200 and 2 400 µg/ml) and adult inhibition of motility assay (0.25, 0.5, 1, 2, 4 and 8 mg/ml). PBS and levamisole (at 0.125 µg/ml in PBS) were used as negative and positive control groups, respectively. Both plant extracts induced anthelmintic effects on the three life-cycle stages of H. contortus and these effects were significantly different when they were compared to the negative control group (PBS) (P<0.05). The effect was dose-dependent on egg hatching and first stage larvae (L1) but not on adult worms. Magnitude of effect was proportional to concentration of plant extracts for egg hatching and L1 but not for adult worms. Besides, the results showed that the D. oliveri stem bark extract was more ovicidal and larvicidal than A. leiocarpus leaves. It is concluded that these two plants do really possess anthelmintic properties.
Key words: Anthelmintic, leaf, stem bark, extracts, Anogeissus leiocarpus, Daniellia oliveri, Haemonchus contortus.