International Journal of Plant Breeding and Genetics

International Journal of Plant Breeding and Genetics ISSN 2756-3847 Vol. 12 (5), pp. 001-008, May, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Evaluating the Hair Regrowth Potential of Botanical Ethanol Extracts Using Cellular and Mouse Models

Bo Huang1, Bueom-Goo Kang2, Zhiqiang Wang2,4 and Soon Sung Lim2,3,4*

1College of Food Science and Engineering, Liaoning Medical University, Jinzhou 121000, China.
2Department of Food Science and Nutrition, College of Natural Sciences, Hallym University, Chuncheon, Gangwon-do,
200-702, Republic of Korea.
3Institute of Natural Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.

4Institute of Korean Nutrition, Hallym University, Chuncheon, 200-702, Republic of Korea.

Received 7 January, 2024; Accepted 16 November, 2024

This study determined the effect of 30% ethanol extract of plant mixture (EPM: Glycyrrhiza uralensis, Angelica gigas, Acorus calamus, Cnidium officinale, Panax ginseng, Camellia sinensis, Salvia miltiorrhiza, Zanthoxylum schinifolium, Carthamus tinctorius, Prunus persica and Scrophularia buergeriana) on promotion of hair growth in human hair dermal papilla cells and C57BL/6J mice. EPM significantly increased the proliferation of human hair dermal papilla cells in a dose- and time-dependent manner (p < 0.05 and p < 0.01). EPM also enhanced mRNA and protein levels of growth factors such as IGF-1, VEGF, KGF and HGF. Moreover, photographical and histological observation showed that application of EPM resulted in the early onset and prolongation of the anagen phase of the hair cycle. In addition, the EPM was revealed to possess potent inhibitory effect on the 5α-reductase activity. Taken together, these results suggest that EPM has hair growth promoting potential and can be used for hair growing products.

Key words: Hair growth, extract of plant mixture, growth factors, steroid 5α-reductase.