ISSN 2756-326X
Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (7), pp. 001-005, July, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
In Vitro Regeneration of the Endangered Kaempferia galanga L.
K.P. Kochuthressia1, S.John Britto2 and M.O. Jaseentha1
11Department of Botany, Carmel College, Mala, Thrissur-680732
2The Rapinat Herbarium and Centre for Molecular Systematics St.Joseph’s College (Autonomous), Tiruchirappalli-
620002
Accepted 06 February, 2024
An efficient protocol is out lined for multiple shoot induction of a medicinally valuable Kaempferia galanga L. using rhizome segment explants. Murashige and Skoog medium supplemented with BA (2.0 mg/ml) and Kn (1.0 mg/ml) exhibited regeneration rate up to 10.85±1.34 shoot/explants. Spontaneous rooting of shoots occurred in the same concentration of cytokinin. The number of leaves (5.21±0.34) and profuse rooting (12.14±1.67) facilitated 100% of plant recovery on acclimation. In vitro derived plants were morphologically identical to the mother plant.
Keywords: Kaempferia galanga, multiplication, regeneration, rhizome segment.